Daytime stress-induced pineal gland structural changes.

Sibarov D.A., Kovalenko R.I.
Daytime stress-induced pineal gland structural changes.
28th Gottingen Neurobiology Conference N1022. (june 7-10), 2001, Gottingen, Germany.

St.Petersburg State University, General Physiology Dep.University emb. 7/9, 199034, St.Petersburg, RUSSIA

In the daytime structural changes of pineal gland during 48-hour water and food deprivation (our stress model) have been detected using optic and fluorescent microscopy. The figure illustrates the changes of total square of different pineal structures in the slice in stress.The shift of light/dark pinealocytes equation to dark cells, decrease of all cells volume, lipid droplets accumulation in the cell body, enlargement of intercellular space and capillars were detected after 48-water and food deprivation. Furthermore, using of acridine orange dyer during fluorescent study revealed stress induced intensification of RNA synthesis in pinealocytes nuclei. We suppose the pineal gland (as an important component of anti-stress system) to increase secretion in stress. RNA accumulation and enlargement of capillars for more effective cell-to-blood excretion confirms this assumption. We have also noticed the lipidization (the process very characteristic for pineal stress changes) and the decrease of pinealocytes volume (probably because of exhaustion during long period of active secretion). It’s in agree with our previously obtained data of electrophysiological and electron-microscopy investigations. Rats also revealed individual differences in the degree of pineal stress changes. It lead us to separate animals into stressed and heavily stressed groups.

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Sibarov D.A., Kovalenko R.I. Daytime stress-induced pineal gland structural changes. 28th Gottingen Neurobiology Conference N1022. (june 7-10), 2001, Gottingen, Germany. St.Petersburg State University, General Physiology Dep.University emb. 7/9, 199034, St.Petersburg, RUSSIA In the daytime structural changes of pineal gland during 48-hour water and food deprivation (our stress model) have been detected using optic and fluorescent microscopy. The figure illustrates the changes of total square of different pineal structures in the slice in stress.The shift of light/dark pinealocytes equation to dark cells, decrease of all cells volume, lipid droplets accumulation in the cell body, enlargement of intercellular space and capillars were detected after 48-water and food deprivation. Furthermore, using of acridine orange dyer during fluorescent study revealed stress induced intensification of RNA synthesis in pinealocytes nuclei. We suppose the pineal gland (as an important component of anti-stress system) to increase secretion in stress. RNA accumulation and enlargement of capillars for more effective cell-to-blood excretion confirms this assumption. We have also noticed the lipidization (the process very characteristic for pineal stress changes) and the decrease of pinealocytes volume (probably because of exhaustion during long period of active secretion). It’s in agree with our previously obtained data of electrophysiological and electron-microscopy investigations. Rats also revealed individual differences in the degree of pineal stress changes. It lead us to separate animals into stressed and heavily stressed groups.