Adenovirus 5 E1A Reagents

Plasmids

  • Plasmids containing the Ad5 E1A 13S or 12S cDNAs used to be available from the ATCC, but don't appear to be listed in the latest catalog. Contact the ATCC for more information.

    Antibodies

    Monoclonal:

  • The M58 antibody developed by Dr. E. Harlow can be obtained from many sources, including:
    abcam
    BD Pharmingen

    Note: This antibody binds in exon 1 between residues 81-139

  • The M73 antibody developed by Dr. E. Harlow can be obtained from many sources, including:
    abcam
    Chemicon
    SantaCruz Biotechnologies
    Sigma

    Note: This antibody binds at the C-terminus of exon 2

  • Phosphospecific polyclonal antisera against Ad2/5 E1A are also available:
    P-Ser 89 SantaCruz Biotechnologies
    P-Ser 219 SantaCruz Biotechnologies

    Cell Lines

  • The 293 human embryonic kidney cell line developed by Dr. F.L. Graham can be obtained from the ATCC as CRL-1573. This cell line constitutively expresses the proteins encoded by E1A and E1B.

    Virus

  • Adenovirus type 5 (VR-5) can be obtained from the ATCC

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