Preston Blair worked with Al Eugster on Krazy Kat cartoons for the Mintz studio. Blair later went to Disney, where he was an animator on "The Sorcerer's Apprentice" and "Dance of the Hours" sequences in Fantasia.

In the '40's, Blair moved to MGM where he worked for Tex Avery. His best known work during that period was Red, first seen in Red Hot Riding Hood and in the cartoons Swingshift Cinderella, The Shooting of Dan McGoo and Red Hot Riding Hood.

Blair co-directed three MGM cartoons with Michael Lah. They are The Bear and the Hare, The Bear and the Bean, and Goggle Fishing Bear.

In later years, Blair worked on many TV commercials and produced a whole episode of the first season of The Flintstones.

Blair was also the author of two books on animation, called Animation and How to Animate Film Cartoons, published by Walter Foster. Both these books continue to be valuable resources for professional animators and animation students.