Methods in molecular genetics


whatever happened and the DNA you prepared is of lousy quality, then use this method!!


DNA extraction procedure II from human blood

for DNA-extraction procedure I "low-cost" click here

for DNA-preparations from specimens of lower quality (heparin-stabilised, older than 5 days, hemolysed), I actually use a commercially available DNA-extraction kit. It provides DNA of good quality and is very fast:

QIAGEN-QIAamp Blood Kit Mini (~200ml of blood) /Midi (~2 ml of blood)





                for an excellent example go here
                         (literature contributed by: skladny-marachelian-niachos)
 
 
 
 

               As the recipe and the working-instruction may be changed by the supplier (QIAGEN),
                I won't give a detailed description.

                Just that much:
                you may use EDTA- or heparin stabilised blood-samples or even amniocytes. Lyse them
                with lysis-buffer and load the whole thing onto the affinity-columns.
                The DNA is bound to the column and washed twice. The elution of DNA is performed
                within 5 to 10 min and gives comparable results to the classical phenol-chloroform method.
                Even older specimens may be extracted succesfully with this method.
 
 







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