Feb. 26, 2001
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Teens influenced by movie star smokers

HANOVER, NEW HAMPSHIRE -- Feb. 26, 2001 -- Teen smokers are more likely to take up smoking if their favorite movie stars smoke, according to new smoking prevention research.

And most of the biggest movie stars used tobacco at least once during the period of the study, with Leonardo DiCaprio, Sharon Stone, and John Travolta clocking the highest number of smoker roles.

A research team led by Jennifer Tickle of Dartmouth College in Hanover, New Hampshire published the survey results in the March issue of the journalTobacco Control by .

"We believe this evidence strongly suggests that media portrayals of tobacco use by popular movie stars contribute to adolescent smoking," said the authors in a prepared statement.

Altogether 632 students, aged 10 to 19, from five rural New England schools took part in the study between 1994 and 1996. At the same time 43 selected movie stars were assessed for on-screen portrayals of smoking.

The students were surveyed about their smoking habits and their attitudes toward smoking. Their responses were scored on a five point scale. The teens were also asked to name their favorite movie star.

Teens whose favorite stars smoked in films were more likely to score higher on the five point scale than teens whose chose stars who did not smoke. And the more a star portrayed smoking, the more favorably the adolescent viewed smoking.

Teens whose favorite stars smoked in two films were 1.5 times more likely to score on the upper end of the scale, while those whose favorite stars smoked in three or more films were three times as likely to do so.

Among teens who had never smoked, those who chose stars who smoked in three or more films were 16 times as likely to express favorable opinions about smoking than those who chose "non-smoking" stars. Stars who smoke may therefore encourage teens to try out smoking, suggest the authors.
    


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 Feb. 26, 2001
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Swallow a gene to treat and prevent cancer? May be possible

26 FEBRUARY 2001-- In a stunning result in laboratory mice, researchers, have for the first time devised a technique to prevent cancer using a therapeutic gene delivered orally.

A team of cancer geneticists and neuroscientists led by Dr. Carlo Croce, director of the Kimmel Cancer Center in Philadelphia, greatly reduced the incidence of stomach cancer in mice bred to be genetically prone to develop tumors, and had been exposed to a cancer-causing substance.

"This is the first time we can prevent cancer by using gene therapy," says Croce in a prepared statement. "The technique could have potential use in several human conditions, such as in precancerous lesions in the lung, esophagus, head and neck, bladder and cervix."

While it's too early to say how effective this may be in humans, the work is another step in the development of safe and effective use of gene therapy for cancer.

Croce and colleagues Drs. Kay Huebner, Matthew During, at Jefferson Medical College and their co-workers report their results in today's online version of the Proceedings of the National Academy of Sciences. The article will appear in the printed journal March 13.

"We were pretty surprised that it worked so well," said Huebner in a prepared statement. "We knew we could kill cancer cells in the laboratory, but we didn't know if the viruses would get eaten up by the stomach juices. We expected differences, but not so dramatic."

In the study, the researchers created 12 genetically altered "knockout" mice, so named because they lack a gene called FHIT, which is normally located on chromosome 3. When damaged, FHIT has already been implicated in contributing to a number of cancers, such as esophageal, stomach, kidney, breast and lung.

Each mouse was exposed to nitrosomethylbenzylamine (NMBA), a carcinogen, beginning four weeks prior to therapy. The scientists' plan was to cause tumors to develop and then try to either prevent or slow their growth with gene therapy.

All of the 12 control mice receiving NMBA developed tumors. Each of eight mice in three therapy groups received one dose of gene therapy with a different type or combination of viral delivery systems. The viruses used are genetically engineered to carry the therapeutic gene into the cell nucleus.

The results clearly showed a difference in the number of tumors. Among the untreated mice, all developed several tumors in the stomach. In the mice treated with adeno-FHIT, 50 percent had tumors. In the AAV mice, only three of eight had tumors. As a result, the researchers believe the AAV delivery method might result in longer expression of the FHIT gene.

"These results are very exciting," During says. "This is one of the first studies to demonstrate a gene transfer that is prophylactic. On the heels of the recent announcement of the sequencing of the human genome and our growing ability in genetic profiling, it's a step toward enabling us to treat high-risk individuals and reduce the likelihood of cancer."

The scientists think the technique may be a novel early stage approach to prevent and perhaps treat cancer.

The scientists say many hurdles still remain to effective and widespread use of gene therapy for cancer, not the least of which is getting the virus into every cell affected. Many regard gene therapy not so much a potential cure but another weapon in the array of treatments aimed at controlling cancer.

Huebner says, the approach needs a lot more study, adding that scientists would have to develop different techniques to deliver genes to other areas of the body, such as the lung, kidney and liver.

One next step for the research team is to develop other animal tumor models with FHIT gene knockouts for both cervical and lung cancer.
    


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