July 13, 2001
     Prostate Cancer

 

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Cold sore virus transformed into a better cancer killer

NEW YORK -- July 13, 2001 (Cancer Digest) -- Scientists have found a way to boost the cancer-killing ability of a genetically modified virus that normally causes cold sores. The research could lead to more potent anti-cancer therapies for prostate and other types of cancers.

New York University School of Medicine researchers report in the July 17 print edition of the Proceedings of the National Academy of Sciences that they have isolated a new version of a herpesvirus that kills cancer cells but spares normal tissue.

Dr. Ian Mohr, assistant professor of microbiology at NYU, who led the research, cautions that the study was done in mice, and no human studies using the new virus have been attempted.

"We took a crippled virus and essentially made it into a more effective killer of cancer cells," he said in a prepared statement. "Clearly the next step is to see whether this more potent anti-tumor virus works in other animal models."

The NYU team injected a new version of the genetically engineered herpesvirus with an added mutation directly into human prostate cancer tumors growing in specially bred mice that had no immune systems. In another group of mice, they injected an earlier version of the herpesvirus, and in the last group they injected an inert solution that didn't contain virus.

Thirty-four days after the treatment, the tumors injected with the new version were, on average, eight times smaller than the tumors injected with the older version of the virus. Moreover, the tumor mass had completely disappeared in up to 40 percent of the animals injected with the new version of the virus.

Dr. Samir Taneja, assistant professor of urology, who is a co-author of the study says the result represents a significant advance developing anti-cancer viruses.

"We think this new virus offers great promise as a therapeutic strategy for the treatment of patients with prostate cancer," he says. "Eventually we hope to test this virus in humans but we still have many things to work out.".

While it has been more than a decade since scientists learned how to create disarmed viruses that target rapidly dividing cells, progress has been stymied by an inability to infect all the cells in a tumor.

The result says Mohr, "is that the virus doesn't completely destroy the tumor mass, and the surviving cancer cells can simply grow."

To solve this problem, Mohr's group observed the effect of the genetically engineered herpesvirus on human cancer cells grown in a laboratory dish. They isolated the viral strain that was the most effective killer of cells and discovered that it contained an extra genetic mutation that enabled more robust reproduction of the virus.

This extra mutation switches the production of a protein called Us11 to an earlier time in the infection cycle of the virus, which prevents the cell from mounting a response to stop viral replication.

Although this kind of viral research is still in its infancy at least 10 different cancer-killing viruses are in early clinical trials or will be soon.

SOURCE: Proc. Natl. Acad. Sci. USA, www.pnas.org/cgi/doi/10.1073/pnas.161011798


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