RESEARCH
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| AUTHORS | Kholodii GYa. Mindlin Sz. Bass IA. Yurieva OV. Minakhina SV. Nikiforov VG. |
| INSTITUTION | Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia. |
| TITLE | Four genes, two ends, and a res region are involved in transposition of Tn5053: a paradigm for a novel family of transposons carrying either a mer operon or an integron. |
| SOURCE | Molecular Microbiology. 17(6):1189-200, 1995 Sep. |
| COUNTRY OF PUBLICATION | |
| ABSTRACT | The complete nucleotide sequence of an 8447 bp-long mercury-resistance trasposon (Tn5053) has been determined. Tn 5053 is composed of two modules: (i) the mercury-resistance module and (ii) the transposition module. The mercury-resistance module carries a mer operon, merRTPFAD, and appears to be a single-ended relic of a transpson closely related to the classical mercury-resistance transposons Tn21 and Tn501. The transposition module of Tn5053 is bounded by 25 bp terminal inverted repeats and contains four genes involved in transposition, i.e. tniA, tniB, tniQ, and tniR Transposition of Tn 5053 occurs via cointegrate formation mediated by the products of the tniABQ genes, following by site-specific cointegrate resolution. This is catalysed by the product of the tniR gene at the res region, which is located upstream of tniR. The same pathway of transposition is used by Tn402 are representatives of a new family of transposable elements, which fall into a recently recognized super-family, and transposons Tn552 and Tn7. We suggest that the tni genes were involved in the dissemination of integrons. |
| PUBLICATION TYPE | Journal Article |
| LANGUAGE | English |
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