Non-Vertigo DC:
Gay/bisexual:
Pied Piper (FLASH [second series] #53) and James (FLASH [second series] #106)
Lesbian/bisexual:
Maggie Sawyer and Toby Raynes (Both in SUPERMAN [second series] #15)
'Ozmagendered' (Ozmagendered = when characters change gender using magic, alien science, or other metaphysical means on a more permanent basis, but only when they have flirtations or sexual encounters with another character while they are in that state; otherwise they are listed as transgendered):
Sir Tristan (CAMELOT 3000 #7) and Isolde
Vertigo:
Gay/bisexual:
Constantine, John (HELLBLAZER #51)
Lesbian/bisexual:
Talbot, Judy (SANDMAN #6); Chantal; Zelda (Both in SANDMAN #11); McNamara, Hazel; and Donna/Foxglove (Both in SANDMAN #32)
Transgendered:
Wanda (SANDMAN #32)
Tasmanian Devil (JUSTICE LEAGUE INTERNATIONAL QUARTERLY #8)
Hero (SUPERBOY AND THE RAVERS #13) and
Leander (SUPERBOY AND THE RAVERS #16)
Josiah Power and Rupe (Both in POWER COMPANY: JOSIAH POWER #1)
Element Lad (LEGION OF SUPER-HEROES [fourth series] #31) and Lightning Lord (LEGION OF SUPER-HEROES [fourth series] ANNUAL #3)
Starman III and Tony (Both in STARMAN [second series] #45)
Berg, Terry (GREEN LANTERN [third series] #137)
Off-Ramp and Frostbite (Both in YOUNG HEROES IN LOVE #1,000,000)
Daly, Samual (BLACK LIGHTNING [second series] #5)
Houston (RELATIVE HEROES #3) and Rive (RELATIVE HEROES #6)
Extrano (MILLENNIUM #2)
Cannon and Sabre (Both in VIGILANTE #5)
Kwin, Arn and Ivar, Xamuel (Both in LEGIONAIRRES #38)
Kevin Mayer (WONDER WOMAN ANNUAL #1) and Oscar
(WONDER WOMAN [second series] #50)
Mantegna, Tony (ACTION COMICS WEEKLY #624)
Sekofsky, Mitch (SUICIDE SQUAD #19)
Hooded Justice and Captain Metropolis (Both in WATCHMEN #9)
Polk, Toland; Noone, Sammy; and Pepper, Lester (All from STUCK RUBBER BABY)
Andy Jones (SUPERGIRL [third series] #10; see also Comet)
Shrinking Violet and Lightning Lass (Both in LEGION OF SUPER-HEROES [fourth series] #1)
Icemaiden II and Olivia Reynolds (Both in JUSTICE LEAGUE AMERICA #110)
Holly (from CATWOMAN)
Lee and Li (Both in GREEN LANTERN [second series] #69)
Syonide and Fauna (Both in OUTSIDERS [second series] #16)
Phillipus (WONDER WOMAN: OUR WORLDS AT WAR #1)
Menalippe and Penelope (WONDER WOMAN [second series] #58)
Iphtime and Anaya (WONDER WOMAN [second series] #168)
Molly Savoy (SOVEREIGN SEVEN #2)
Silhouette (WATCHMEN #2)
Comet (SUPERGIRL #25)
Burgess, Alex; McGuire, Paul (Both in SANDMAN #1); Carter, Hal; Robert (SANDMAN #15); Cluracan (SANDMAN #27)
Enigma, from the series of the same name (Also featuring Michael Smith and Titus Bird)
Go-Go Fiasco (CODENAME KNOCKOUT #0)
Monsieur Mallah and the Brain (Both in DOOM PATROL [second series] #34)
Mowbray, Charles and Jeremy (Both in HEARTTHROBS #1)
Bridges, Detective Paulie
Sebastian O, from the series of the same name
Ray Monde (HELLBLAZER #6)
Uncle Gaylord and Mr. Dimhoffer (from MOONSHADOW)
Ernesto (Back-up story in HOUSE OF SECRETS #15)
Leonardo Da Vinci and Salai, both from the CHIAROSCURO mini-series
David Wojnarowicz (from SEVEN MILES A SECOND)
George, Kathy (SHADE, THE CHANGING MAN #20) and Shapiro, Lenny (SHADE, THE CHANGING MAN #8)
Liz Tremayne (from SWAMP THING)
Jolly Roger (INVISIBLES [second series] #1)
Myra and Maxine (Both in ANIMAL MAN #65)
Harkness, George; and Phoebe (Both in SEBASTION O #2)
Lord Fanny (INVISIBLES [first series] #2)
Echo (DREAMING #17)