HIGH PERFORMANCE OPTICAL ABSORBANCE DETECTORS BASED ON LOW NOISE SWITCHED INTEGRATORS

(Talanta, 1993 40, 1331-1338)

HANGHUI LIU, PURNENDU K. DASGUPTA and HONG J. ZHENG

Department of Chemistry and Biochemistry

Texas Tech University, Lubbock, TX 79409-1061

(Reviewers' Comments)

Summary

Optical absorption detection is the most common analytical measurement principle in liquid phase analysis. The current state-of-the-art of commercially available detectors exhibit peak-to-peak (p-p) noise levels in the range of 1 x 10^-5 - 2 x 10^-5 absorbance units (10-20 uAU). Using circuitry based on newly available switched integrator integrated circuit (IC) packages, it is possible to construct inexpensive absorbance detectors with p-p noise levels as low as 3 uAU under actual use conditions. The necessary electronics is described and performance data are reported with light emitting diodes (LEDs) as light sources. Even in the capillary format with a rectangular capillary (50 x 1000 um cross section) with a slitwidth <50 um and with the 1000 um dimension as the nominal pathlength, p-p noise levels of 10 uAU are observed, from which a concentration limit of detection (LOD) of 10 nM for bromothymol blue (BTB) can be estimated with a 660 nm light source.


1