Table 4.

Effects of cold stress on floral morphology, anther dehiscence and esterase activity (±SE) in chickpea cultivars/lines grown in growth rooms.
 
Temperature
(° C)
Cultivar/line
Annigeri ICCV
88502
ICCV
88503
Pant G 114 ICCV
88510
ICCV
88514
 
Duration from bud appearance to flower opening (days)a
25/15 5.6±0.16 6.1±0.18 5.8±0.15 6.0±0.16 6.1±0.18 6.1±0.21
15/5 7.2±0.21 6.7±0.17 6.4±0.17 6.3±0.13 6.4±0.19 6.7±0.17
15/0 8.2±0.19 6.7±0.21 6.6±0.17 7.2±0.15 7.0±0.18 7.2±0.19
 
Width of vexillum (mm)
25/15 6.5±0.21 6.8±0.21 6.7±0.25 7.1±0.23 6.8±0.21 7.0±0.20
15/5 6.1±0.20 6.5±0.21 6.7±0.21 7.2±0.19 6.7±0.22 7.1±0.17
15/0 6.3±0.24 6.5±0.23 6.8±0.24 7.2±0.21 7.0±0.19 6.7±0.21
 
Length of style (mm)
25/15 6.4±0.18 6.6±0.21 6.4±0.22 6.9±0.19 6.5±0.22 6.7±0.16
15/5 5.7±0.19 6.5±0.17 6.2±0.18 6.1±0.21 6.6±0.11 6.7±0.19
15/0 4.4±0.16 6.3±0.19 6.3±0.19 5.7±0.22 5.9±0.14 6.4±0.18
 
Length of ovary (mm)
25/15 1.9±0.07 2.0±0.08 1.8±0.07 2.0±0.07 1.9±0.08 1.9±0.07
15/5 1.4±0.06 2.1±0.08 1.9±0.06 2.0±0.06 2.0±0.07 1.8±0.07
15/0 1.3±0.06 1.9±0.07 1.8±0.05 1.9±0.07 1.9±0.07 2.0±0.08
 
Flowers with fully dehiscing anthers (%)
25/15 100(17/17)b 100(15/15) 100(14/14) 100(15/15) 100(15/15) 100(16/16)
15/5 71(11/17) 93(14/15) 94(16/17) 87(13/15) 88(14/16) 88(15/17)
15/0 35( 6/17) 75(12/16) 81(13/16) 69(11/16) 76(13/17) 82(14/17)
 
Partially dehiscent or indehiscent anthers per 10 flowers (no.)
25/15 0 0 0 0 0 0
15/5 26 5 4 7 8 6
15/0 41 9 7 19 15 12
 
Flowers with pollen on stigma (%)
25/15 100(17/17) 100(15/15) 100(14/14) 100(15/15) 100(15/15) 100(16/16)
15/5 65(11/17) 93(14/15) 100(17/17) 100(15/15) 88(14/16) 88(15/17)
15/0 18( 3/17) 81(13/16) 88(14/16) 69(11/16) 71(12/17) 65(11/17)
 
Esterase activity on stigmac
25/15 4.8±0.07 4.9±0.08 4.8±0.08 4.9±0.07 4.8±0.08 4.9±0.07
15/5 2.3±0.19 4.1±0.18 4.0±0.15 4.2±0.18 3.9±0.15 4.1±0.11
15/0 1.6±0.15 3.8±0.21 3.8±0.17 3.2±0.16 3.4±0.17 3.2±0.16

aThe buds, which failed to open especially in the 15/0oC regime, were not considered in calculations.
bFigures in parentheses: (flowers with only dehiscing anthers or with pollen on stigma/total number of flowers tested)
cEsterase activity was scored on a 1-5 scale (1, low and 5, high) 1 day before flower opening.
Note : Cultivar/line means were significantly different according to the Kruskal-Wallis test.
 
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