Renganathan Kutralanathan

Kutralanathan Renganathan

Case Western Reserve University / Cleveland Clinic

9500 Euclid Ave., Cleveland, OH 44195

EDUCATION:

· Ph.D. Chemistry, Case Western Reserve University, Cleveland, OH, July 2001 – 2006 (Projected July 06)

· M.S. Chemistry, Eastern Michigan University, Ypsilanti, MI, June 2001

· M.Sc. Chemistry, Indian Institute of Technology Madras (IIT Madras), India, June 1998

· B.Sc. Chemistry, Loyola College, Chennai, India, June 1996.

RESEARCH EXPERIENCE:

Case Western Reserve University and Cleveland Clinic, Cleveland, OH July 2001 to Present

Advisors: Dr. John W Crabb (Cleveland Clinic)

Dr. Robert Salomon (Case Western Reserve University)

Co-Advisor: Dr. Bela Anand-Apte (Cleveland Clinic)

Dissertation title: Role of Lipid Peroxidation in Retinal diseases

· Cell culture investigation of the role of carboxyethylpyrrole (CEP), a docosahexaenoate derived unique protein modification, in stimulating angiogenesis, implicating its importance in Age Related Macular Degeneration (AMD).

· Characterized retinal protein adducts and retinal protein expression after intense blue and green light treatment on rats for understanding the molecular mechanisms leading to retinal light damage. Identified oxidatively modified (CEP, argpyrimidine, and nitrotyrosine) proteins using 2D electrophoresis followed by mass spectrometric analysis.

· Screened human plasma samples by ELISA for CEP and ethylpyrrole, protein modification as possible biomarkers for AMD

Eastern Michigan University, Ypsilanti, MI Jan 1999 to Jun 2001

Advisor: Dr. Deborah Heyl-Clegg

Dissertation title: Aromatic Amino Acid Replacement at the N-Terminus of a Nonselective Opioid Deltorphin Analog: A QSAR Study

· Synthesized deltorphin peptides (Tyr-D-Ala-Phe-Gly-Val-Val-Gly-NH₂) by replacing the position one tyrosine (Tyr) with analogues of varying electronic, steric, and acid/base character, including ring-substituted tyrosines, para-substituted phenylalanines, and other nonaromatic and heterocyclic amino acids. The synthesized peptides were purified by HPLC and purity was verified with mass spectrometry.

· Quantitative Structure Activity Relationship (QSAR) was done on the binding data to understand the role of phenolic hydroxyl group in mu and delta opioid receptor binding.

Indian Institute of Technology Madras, Chennai India July 1996 to Jun 1999

· Synthesized, purified and characterized Tetraphenylporphyrin.

TEACHING EXPERIENCE:

· Lab Teaching assistant, Winter 1999 - Summer 2001, Department of Chemistry, Eastern Michigan University

Chemistry 101 - Chemistry for Elementary Teachers Jan 1999 – Aug 1999

Chemistry 116 - Chemistry and Society Laboratory Jan 1999 – Aug 1999

Chemistry 118 - Fundamentals of Chemistry Laboratory Jan 1999 – Aug 1999

Chemistry 122 - General Chemistry I Laboratory Sep 1999 – Dec 2000

Chemistry 124 - General Chemistry II Laboratory Jan 2000 – Aug 2000

Chemistry 271 - Organic Chemistry Laboratory Jan 2001 – May 2001

· Lab Teaching assistant, Fall 2001 and Spring 2002, Department of Chemistry, Case Western Reserve University

CHEM 331 - LAB Methods & Techniques III Sep 2001 – Dec 2001

CHEM 113- Principles of Chemistry Lab Jan 2002 – May 2002

PROFESSIONAL SOCIETY AFFILIATIONS:

The American Chemical Society (ACS), The Association for Vision Research and Ophthalmology (ARVO), American Association for the Advancement of Science (AAAS), The American Oil Chemists’ Society (AOCS)

SKILLS:

· Immunological techniques: Western blot analysis, immunoprecipitation (IP) and enzyme-linked immunosorbent assays (ELISA)

· Analytical techniques: NMR spectroscopy, UV spectroscopy, Phenylthiocarbamyl amino acid analysis (PTC) and mass spectrometry (Applied Biosystems (ABI) Voyager MALDI-ToF , Waters Q-ToF-2, ABI PE Sciex API 3000 Triple-Quadruple, and ABI 4800 MALDI-TOF-TOF analyzer).

· Cell Culture: Experience in ARPE-19, RPE-J, D407, and Monkey endothelial cell lines

· Synthesis of Peptides (FMOC and BOC methods) – Both by manual step wise addition and by automated Peptide synthesizer (PS3 Automated Peptide Synthesizer from Protein Technologies)

· Protein purification using HPLC (Agilent Capillary LC 1100 series and Waters HPLC systems), SDS-PAGE (1D & 2D), solution state isoelectric focusing (multi-compartment electrophoresis MCE-IEF)

· Lipid and Protein Extractions from rat and mouse retinas

· Computer programming - Basic, UNIX, C, C++, JAVA, ORACLE and HTML.

PUBLICATIONS:

Heyl DL, Sanvordekar MD, Dogruyol G, Salamoun MD, Rodgers DW, Renganathan K, Mousigian C and Schullery SE. (1999) Synthesis of new group of Deltorphin I/II analogs modified in the address domain with -amino acids, and QSAR study of their opioid binding, Protein and Peptide Letters, 6, 359-366.

Schullery SE, Rodgers DW, Tripathy S, Jayamaha DE, Sanvordekar MD, Renganathan K, Mousigian C, Heyl DL. (2000) Support for the Conformational Role of the Val5-Val6 Residues of Deltorphins II: A QSAR Study of New Analogues Modified in the Address Domain, Bioorg Med Chem. 9, 10, 2633-2642.

Heyl DL, Schullery SE, Renganathan K, Jayamaha MN, Rodgers DW, Traynor JR (2003) pKa and volume of residue one influence delta/mu opioid binding: QSAR analysis of tyrosine replacement in a nonselective deltorphin analogue, Bioorg Med Chem. 2003 Aug 15;11(17):3761-8.

Bhattacharya SK, West KA, Gu X, Crabb JS, Renganathan K, Wu Z, Sun J and Crabb JW (2004) “Fractionation of Retina for Proteomic Analyses” In: Separation Methods In Proteomics (GB Smejkal and A Lazarev, eds), Marcel Dekker, New York (In Press).

Renganathan K, Ebrahem Q, Vasanji A, Gu X, Salomon RG, Crabb JW, and Anand-Apte B (2006) Carboxyethylpyrrole Oxidative Protein Modifications in Age-Related Macular Degeneration Stimulate Angiogenesis. PNAS in review.

Renganathan K, Gu X, Grimm C, Wenzel A, Salomon RG, Reme CE and Crabb JW. (2006) Rapid Changes in Retinal Oxidative Protein Modifications Induced by Blue Light. In preparation

Gu X, Renganathan K, Darrow R, Gu J, Collier R, Kapin M, Hollyfield JG, Salomon RG, Organisciak D and Crabb JW Similarities in Oxidative Damage from AMD and Retinal Light Damage (2006). In preparation

PRESENTATIONS:

Renganathan K, Jayamaha MN, Traynor J, Schullery SE, Heyl DL. (2000) Aromatic Amino Acid Replacement at the N-Terminus of a Nonselective Opioid Deltorphin Analog: A QSAR Study, CMACS 2000, Abstract 140314.

Renganathan K, Sun M, Darrow R, Shan L, Gu X, Salomon RG, Hazen S, Organisciak D and Crabb JW. (2003) Light Induced Protein Modifications and Lipid Oxidation Products in Rat Retina. Annual Meeting of the Association for Research in Vision and Ophthalmology, May 4-9, 2003, Ft. Lauderdale, FL, Abstract 5129.

Renganathan K, Ebrahem Q, Gu X, Salomon RG, Anand-Apte B and Crabb JW. (2003) Carboxyethylpyrrole Protein Modifications Stimulate Angiogenesis. Great Lakes Vision Research Conference, Miami University, Oxford OH, October 17-18, 2003.

Gu X, Renganathan K, Grimm C, Wenzel A, Salomon RG, Reme CE and Crabb JW. (2004) Rapid Changes in Retinal Oxidative Protein Modifications Induced by Blue Light. Annual Meeting of the Association for Research in Vision and Ophthalmology, April 25-29, 2004, Ft. Lauderdale, FL. Abstract 3474.

Renganathan K, Collier R, Gu X, Salomon RG, Kapin M, Hollyfield JG and Crabb JW. (2004) Similarities in Oxidative Damage from AMD and Retinal Light Damage. Annual Meeting of the Association for Research in Vision and Ophthalmology, April 25-29, 2004, Ft. Lauderdale, FL. Abstract 1795.

Renganathan K, Gu J, Gugiu B, Gu X, Darrow R, Lewis H, Salomon RG, Organisciak DT, Crabb JW. (2005) Similarities in Plasma Oxidative Modifications From Retinal Light Damage and AMD. Annual Meeting of the Association for Research in Vision and Ophthalmology, May 1-5, 2005, Ft. Lauderdale, FL. Abstract 3010.

Crabb JS, Renganathan K, Gu X, West KA, Bhattacharya SK, Wu Z, Crabb JW. (2005) Fractionation of Retina for Proteomic Analysis. Annual Meeting of the Association for Research in Vision and Ophthalmology, April 25-29, 2004, Ft. Lauderdale, FL. Abstract 5154.