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My email: blindr01@popmail.med.nyu.edu, or you can just click the "Send me an email" button below. You can add me to your AOL Instant Message buddy list by clicking here, or go to the AOL IM banner at the bottom of the page. NANK.
This is me and Tony Kim, another graduate student in Yossi's lab, in a paddle boat at the Skirball retreat at the Mohonk resort in Mohonk, NY. What a place. Go visit if you can. This Korean guy is a real stud. He's a PhD student at NYU. Uh, a real find... Email him HERE
GRITTY 
GUTTY 
The Best Sabres Sites:
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tsn.ca is the best site for everything NHL, and ALWAYS has the first word on any trades and other transaction information. I dont know how they do it, but they always get the information out there first.
Slam Sports in canada has probably the best all around coverage you can find, from rumors to stats. They usually have some new news everyday on the teams, other than game results.
Listen to Sabres games streaming LIVE on empiresports RealAudio using RealPlayer. Its better than nothing. You can also tune in to hear Roby and Brian Blessing on Labatt Hockey Hotline after the game.
Empire Sports network Sabres summary is updated after every period of every Sabres Game. Gives you a feel for how the team is performing in the form of a summary.
USAToday NHL coverage has game updates every 5 minutes, probably the best site for the latest breaking NHL transaction information.
ESPN and their Live Game Simulcasts. The simulcast is amazing: it gives you all shots, where they were taken from on the ice, what kind of shot was taken, and tons of other live streaming game info. The very best place to go when you can't listen to the games on RealAudio. Also great to get more in-depth information on attack zone time and where shots are taken during a game (quality chances versus a shot from the red line). I'd have it on while I was watching a game.
The Sporting News and their weekly updated Sabres Team Report. Probably the least infomative site.
METRIC CONVERSION PAGE... Instantly converts all units of measure!!!
So far so good. My thesis project is to solve the X-ray crystal strucutre of the epidermal growth factor receptor extracellular ligand binding domain complexed with TGFa. I am expressing a recombinant construct of domain III in SF9 lepidopteran cells using the baculovirus expression system (Gibco BRL's pFastBac). I have the purity right now @ 95% by just two purification steps, one antibody affinity column and a cation-exchange Mono S. I also generated 25 mutant constructs to attempt to remove the sugar residues from my protein by site directed mutagenesis of N to either D or Q. Someone just recently characterized the glycosylation pattern of EGFR, making my life a little easier. I am also using PNGase F to enzymatically remove the sugars, but I think that some sort of combination of both methods will yield material that will both crystallize and diffract to atomic resolution. I'm going to try to crystallize the fully glycosylated material as well. I'm in the process of setting up crytallizations now and am making new constructs to express my protein in drosophila (S2) cells (these are the cells they used for the HIV-1 envelope glycoprotein gp120 structure). My protein is very stable and is sharp on Mono S. Any suggestions? Email me and I certainly will get back to you.
Just an update: I now have very small phase seperations in my domain 3 construct crystallization screens. I have been told that I need to simply raise the [protein] and I can get nice crystals. That would be nice. I have started working on a domain 123 construct and have generated mutants of that construct as well.
I am now trying to refold the receptor from E.Coli inclusion bodies, since I think the glycosylation is more of a problem than I has originally expected. I have had some success, but the yield is quite low (only about 100 ug/ liter), and I lack a good affinity column. I've been using cation-exchange to seperate native from non-native, which constrains my salt concentrations during refolding. But, I'm still trying.
I am now trying to express EGF in yeast to get a good affinity column that I can use in high salt. Here goes!!!
I am now trying to refold EGF from E.coli to get that good affinity column. The yeast was terrible to work with. My whole lab stunk for the two weeks I worked with it before I scrapped it. EGF was originally soluble when I expressed it, but I was able to push it into inclusion bodies. The refolding should be pretty straight-forward, I hope. I'll let you know when I get it. If I do, I might quit my lab and sell EGF for $50 per microgram. I'd be rich.
